Cytokine gene transfer may provide a powerful strategy for gene therapy of cancer. The effectiveness has been demonstrated in some animal model systems. 1,2 A safe and efficient method is necessary ...

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...

To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline ...

Using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and significantly improves the system's throughput over the ...

Gene-based immunotherapy for cancer is limited by the lack of safe, efficient, reproducible, and titratable delivery methods. Direct injection of DNA into tissue, although safer than viral vectors, ...

Genetic disorders such as cystic fibrosis and long QT syndrome are caused by mutations in human ion channel and ion transporter genes. Electroporation is a non-viral transfection technique ...

Unleash the power of electroporation with the Invitrogen Neon NxT Electroporation System. Join us for an insightful webinar where we demystify the basics of electroporation and guide you on how to ...

Thermo Fisher Scientific’s new Gibco CTS Xenon Electroporation System aims to provide easier scale up for cell therapies, from clinical development to commercial manufacturing. Thermo Fisher ...

Are low gene editing efficiency or gene expression levels preventing your research from advancing? Is your transfection method killing your cells? Are you struggling to reproduce your initial results ...